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Industrial Ozone Solutions.

Advanced Oxidation Sanitizing (AOS)

Up to 50% Less PAA Real-Time UVC Validation No Added Cycle Time Lower COD to Drain Independently Tested FDA · USDA · NSF Aligned

Advanced Oxidation Sanitizing (AOS) · Clean-In-Place for Food & Beverage Processing

Protect Your Brand. Protect Your Product. One CIP Upgrade.

Cut PAA use up to 50%, extend your sanitizer step without adding time, and validate concentration in real time with UVC — independently tested and proven in a working dairy plant.

Advanced Oxidation Sanitizing (AOS) · Clean-In-Place for Food & Beverage Processing

Protect Your Brand. Protect Your Product. One CIP Upgrade.

Cut PAA use up to 50%, extend your sanitizer step without adding time, and validate concentration in real time with UVC — independently tested and proven in a working dairy plant.

WHAT CONVENTIONAL CIP IS COSTING YOUR OPERATION

Your CIP system has a food safety, product loss, and carbon problem that's getting harder to ignore.

Most facilities measure chemical cost. Far fewer have fully quantified the impact of heating water, post-pasteurization contamination, and spoilage — or the organic load those chemicals add to every drain event.

WHAT CONVENTIONAL CIP IS COSTING YOUR OPERATION

Your CIP system has a food safety, product loss, and carbon problem that's getting harder to ignore.

Most facilities measure chemical cost. Far fewer have fully quantified the impact of heating water, post-pasteurization contamination, and spoilage — or the organic load those chemicals add to every drain event.

Thermal Energy at Scale

Conventional CIP operates at 70–85°C. Heating water to those temperatures across hundreds of annual cycles is one of the largest single energy consumers on a processing site — and a direct Scope 1
emission source.

Spoilage Starts after Pasteurization

Between 50 and 60% of fluid milk that spoils microbiologically does so from post-pasteurization contamination — not from the raw milk. Fewer than one cfu/mL of Pseudomonas is enough to breach the PMO limit within four to seven days.

Biofilm Builds at Refrigeration Temperature

Around 72% of dairy Pseudomonas strains form biofilm at 6 °C, and Pseudomonas has been shown to metabolize the very sanitizing compounds aimed at it. Biofilm is not a hypothetical risk; it is the documented source of most dairy spoilage.

COD Loading in CIP Effluent

Residual caustic and organic soils drive up COD in your drain streams. But there is a less obvious contributor that many facilities aren’t measuring — one directly tied to PAA-based sanitation.

PAA blends add organic load — and clouds the numbers you measure.

Commercial PAA blends contain acetic acid and produce more as they decompose: real, biodegradable organic carbon in your effluent.

Residual PAA and hydrogen peroxide also interfere with standard COD and BOD tests, so the readings you're working from may not reflect true load.

Ozone decomposes to oxygen. No organic carbon, no acetic acid, no test interference.

Kitis, Environment International 30:47–55 (2004); Leite et al., Water Science & Technology 84(5):1270–1279 (2021); Sousa et al., Journal of Environmental Engineering 150(1) (2024).
Kitis, Environment International 30:47–55 (2004); Leite et al., Water Science & Technology 84(5):1270–1279 (2021); Sousa et al., Journal of Environmental Engineering 150(1) (2024).

A NEW CLASS OF CIP

Using AOS, we achieve validated sanitation outcomes at temperatures your current chemistry requires heat to reach.

The specific mechanism is patent-pending and shared only under NDA with prospective partners. What the evidence shows: effective kill rates at significantly reduced wash temperatures, substantially reduced chemical volumes, and effluent that is measurably cleaner before it ever reaches your drain.

A NEW CLASS OF CIP

Using AOS, we achieve validated sanitation outcomes at temperatures your current chemistry requires heat to reach.

The specific mechanism is patent-pending and shared only under NDA with prospective partners. What the evidence shows: effective kill rates at significantly reduced wash temperatures, substantially reduced chemical volumes, and effluent that is measurably cleaner before it ever reaches your drain.

Stronger Microbial Risk Mitigation

Ozone attacks biofilm and surface residue directly. Blended ozone + PAA removes more biofilm — confirmed by microbial  monitoring. You clean better, not just cheaper.

Lower CIP Temperatures

Peer-reviewed ozone CIP literature demonstrates effective sanitation at or near ambient temperatures — eliminating the thermal energy demand of conventional hot wash cycles.

🛈 Published Research

Longer contact time, from time already spent

Dose ozone in the pre-rinse and pump-down post-rinse. Turns non-CIP sanitizing and cleaning time into active sanitizing and chemical-residual-removal time — no added cycle length.

Less Chemistry, Less Handling

45–50% reduction in PAA feed-pump time in validated dairy trials. If your CIP uses chlorinated chemistry, fewer chlorinated byproducts — THMs, HAAs, nitrosamines.

Cleaner Effluent - No Acetic Acid Load

Ozone decomposes to oxygen — no organic residual, no acetic acid, no additional COD contribution from the sanitation agent itself entering your drain stream.

Reduced CO₂e,
Scope 1 & 3

Less thermal energy per cycle. Fewer drums manufactured, filled, and shipped. Both are quantifiable, auditable reductions reportable under GHG Protocol, CDP, GRI, or CSRD frameworks.

🛈 Published Research

How AOS Fits Your Existing CIP

How AOS Fits Your Existing CIP

4-Step Design Method

Assess the CIP System

Single-tank single-use · 2-tank reuse · 3-tank designated reuse · rinse recovery

Determine the System Volume and Where Ozone is Introduced

Typically, the pre-rinse and the pump-down post-rinse/final rinse. Volume of each circuit sets the dose.

Determine Ozone Demand

Driven by incoming water characteristics — hardness, organics, temperature, iron and manganese.

Design the Ozone System

Size the cell stack — typically 20–40 cells — against the estimated demand.

The AOS skid is a side-stream addition — your tanks, pumps, valves and PLC sequence stay exactly as they are.

THIS IS NOT A SUBSTITUTION STORY

AOS is not a cheaper way to reach the same result — it reaches a better result, on less chemical, in the same cycle.

THIS IS NOT A SUBSTITUTION STORY

AOS is not a cheaper way to reach the same result — it reaches a better result, on less chemical, in the same cycle.

Ozone + PAA Outperforms PAA Alone

cfu/mL Remaining · Log Scale · Lower is Better
10,000
1,000
100
10
1
2,800.0
44.0
5.5
5 mins
2,733.0
29.5
3.0
10 mins
2,600.0
17.5
3.0
15 mins
Untreated Control
200 ppm Blend
125 ppm Blend + 2 ppm Ozone

6–10× fewer surviving organisms  on 37.5% less sanitizer — consistent at every contact time. Roughly one additional log reduction. B. cereus is a spore-former and among the most resistant dairy biofilm organisms.

Who Tested It, and How

  • BioFoodTech, Charlottetown PE - a Standards Council of Canada accredited, provincially-owned food laboratory (not vendor data)
  • 316 food-grade stainless coupons - real CIP contact surface
  • Commercial 5% PAA/H₂O₂ concentrate — supplied by a working dairy rather than a lab reagent
  • ATCC reference strains — traceable and reproducible
  • Triplicate plating and duplicate runs — results since reproduced

Across the Other Test Organisms

  • S. aureus  ATCC 43300
  • P. aeruginosa  ATCC 27853

25 ppm blend + 2 ppm ozone matched 200 ppm blend — an 87.5% reduction in sanitizer at equal efficacy.

Laboratory biofilms are single-organism; in-plant biofilms are mixed-species and may respond differently — in-plant validation is underway.

WHAT THE EVIDENCE SHOWS

First AOS dairy trial proven on the raw side.

The electrolytic ozone in Clean-In-Place was validated at a fluid-milk facility on the raw-milk circuit. It was verified via inline probe validation in which actual ozone ppm was measured in-line and continuously verified at the point of use, not inferred from generator output or calculated dose.

WHAT THE EVIDENCE SHOWS

First AOS dairy trial proven on the raw side.

The electrolytic ozone in Clean-In-Place was validated at a fluid-milk facility on the raw-milk circuit. It was verified via inline probe validation in which actual ozone ppm was measured in-line and continuously verified at the point of use, not inferred from generator output or calculated dose.

Up to 50%

PAA Reduced
Peracetic acid concentration reduced by half while cleaning performance
was maintained.

<2 ppm

Ozone Held, No Rinse
Ozone no-rinse concentrations kept consistently below the 2 ppm no-rinse threshold.

+3-4 min

Longer Sanitation,
Same Cycle
Ozone added to the pump-down ahead of the final no-rinse sanitize extends sanitation 3-4 min without extending overall CIP time.

0 Impact

Safe Work Environment
Air monitoring showed no negative impact to the workspace throughout the trial.

0 Change

Raw Milk Unaffected
No adverse effect on raw-milk quality — microbial or compositional properties.

On the PAA/acetic acid wastewater interaction (peer-reviewed): Multiple peer-reviewed studies confirm that PAA commercial solutions contain acetic acid as a formulation component, and that PAA decomposition produces additional acetic acid — both contributing measurable COD to effluent. This is compounded when combined with organic soils in food processing CIP drain streams. Ozone’s decomposition pathway (O₃ → O₂) produces no organic carbon and therefore no acetic acid COD contribution.

Kitis, Environment International 30:47–55 (2004); Leite et al., Water Science & Technology 84(5):1270–1279 (2021); Sousa et al., Journal of Environmental Engineering 150(1) (2024).
Kitis, Environment International 30:47–55 (2004); Leite et al., Water Science & Technology 84(5):1270–1279 (2021); Sousa et al., Journal of Environmental Engineering 150(1) (2024).
Real-Time Validation at Point of Use

Real-time measurement closes a food-safety records gap. Manual titration can be missed or mis-entered. Continuous inline measurement creates an objective record of what was actually delivered.

Ozone Verified at Point of Use

Actual ppm measured in-line and continuously — not inferred from generator output or calculated dose.

Proof the PAA was actually delivered.

Non-conductive PAA products cannot be confirmed by conductivity. Inline probes close a validation gap standard CIP cannot see.

Built to Pay for Itself — Without Capital Outlay

No Capital Outlay

The skid is leased, installed and fully maintained. Operates under a fixed monthly cost, not equipment you buy upfront.

Self-Funded by Design

Structured to be offset by chemical, water, energy and logistics savings.

Proven in a Real Plant

Validated at a fluid-milk facility across raw silos, cream tanks, a blender line and truck wash.

Does this sound like your facility?

If you’re actively working on any of these, we’d like to hear from you.
  • Reducing Scope 1 thermal energy in processing
    🛈 Published Research
  • Lowering COD load in wastewater discharge
  • Meeting net-zero or science-based targets
  • Generating ESG-reportable, auditable reduction data
  • Reducing Scope 3 upstream chemical emissions
  • Investigating PAA-related COD surcharge exposure
  • Reducing chemical procurement & logistics burden
  • Simplifying hazardous chemical handling on site
Get In Touch

Reach out to access our cost-savings calculator! Tell us a little about your facility and what you’re working on.

Note: Technical details are shared only under mutual NDA.

Frequently Asked Questions


Conventional hot CIP operates at 70–85°C. The energy required to heat water to those temperatures — repeated across every circuit, every shift, every year — is a meaningful and often underreported Scope 1 emission source. Moving to ambient or significantly lower temperatures removes that thermal demand entirely. It also eliminates the cool-down phase, which has secondary water and time implications.

🛈 Published Research

PAA is sold commercially as an equilibrium mixture that already contains acetic acid as a formulation component, alongside PAA itself and hydrogen peroxide. When PAA decomposes after application, it produces additional acetic acid as a breakdown product. Acetic acid is an organic compound with significant chemical oxygen demand. When this combines with the organic soil load already mobilized during a CIP clean, the resulting effluent COD can be substantially higher than facilities expect — and is often higher than measurements taken before the sanitizer step.

Ozone’s decomposition pathway produces only oxygen. There is no organic residual, no acetic acid, and no additional COD contribution from the sanitation agent itself. This is confirmed by peer-reviewed literature including Leite et al. (Water Science & Technology, 2021) and Kitis (Environment International, 2004).

We work with trial partners to establish a verifiable baseline — measuring thermal energy per CIP cycle, chemical volume consumed, and chemical origin for Scope 3 calculation. We then measure the same metrics under our process. The delta is calculated using standard GHG Protocol methodology, giving you auditable, independently verifiable figures. These can feed directly into CDP, GRI, or CSRD reporting frameworks. We provide a complete data package as part of any trial engagement.

Yes — and this is central to our value proposition. We do not ask you to trade efficacy for sustainability. Peer-reviewed research has demonstrated that aqueous ozone at appropriate concentration and contact time achieves effective pathogen reduction and biofilm removal at ambient temperatures. One published study (Tirpanci Sivri et al., Frontiers in Microbiology, 2023) found that 10 ppm aqueous ozone for 10 minutes reduced Pseudomonas fluorescens biofilm on pilot-scale equipment below the recovery method’s detection limit. The specific validated performance data for our process — concentration, contact time, kill rate, and substrate type — is shared with partners under NDA, alongside independent laboratory validation.

Yes. The FDA affirmed ozone as Generally Recognized As Safe (GRAS) for direct contact with food under 21 CFR 184.1563. The USDA has approved ozone use in federally inspected meat and poultry facilities. Ozone is also recognized as a Best Available Technique (BAT) for CIP in European food processing reference documents (BREFs). Our trial program is structured to generate data fully aligned with regulatory submission and HACCP documentation requirements.

We begin with a 30-minute discovery call — no technical detail shared at this stage, no obligation. If there’s a potential fit, we execute a mutual NDA and conduct a site assessment to confirm compatibility with your existing CIP infrastructure. Trial installation and commissioning is conducted at our cost. We ask for your team’s time during monitoring, and the opportunity to document outcomes — always subject to your written approval before any external publication. There is no obligation to purchase or commit to any commercial arrangement following the trial.

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