Science and Evidence.
Test Results, Certifications, and Case Studies
Explore the science, certifications, and real-world results behind tersano's technology — from independent lab testing and third-party validations to customer success stories across industries.
Pathogen Summary
| Microorganism | Reduction | Dwell Time | Concentration | Test Standard |
| E. coli | 99.999% | 30 secs | 0.3 ppm | AOAC 960.09 |
| Staph | 99.999% | 30 secs | 0.3 ppm | AOAC 960.09 |
| Listeria | 99.999% | 30 secs | 1.0 ppm | AOAC 960.09 |
| Salmonella | 99.9% | 30 secs | 0.3 ppm | ASTM E1153 |
| Microorganism | Reduction | Dwell Time | Concentration | Test Standard |
| Coronavirus SARS-CoV-2 | 99.9% | 10 secs | 0.75 ppm | Academic |
| Poliovirus 1 | 99.9% | 1 min | 0.37 ppm | Academic |
| Hepatitis A | 99.999% | 30 secs | 1.0 ppm | Academic |
| Microorganism | Reduction | Dwell Time | Concentration | Test Standard |
| A. brasiliensis | 99.9% | 15 mins | 0.3 ppm | BS EN 13697 |
| Candida albicans | 99.9% | 15 mins | 0.3 ppm | BS EN 13697 |
| Proteus mirabilis (odors) | 99% | 30 secs | 0.3 ppm | Fabric Surface Sanitizer Method |
| Microorganism | Reduction | Dwell Time | Concentration | Test Standard |
| Enterococcus hirae | 99.999% | 1 min | 1.0 ppm | EN 1276 |
| E. coli | 99.999% | 1 min | 1.0 ppm | EN 1276 |
| Pseudomonas aeruginosa | 99.999% | 1 min | 1.0 ppm | EN1276 |
| Staph | 99.999% | 1 min | 1.0 ppm | EN 1276 |
| Candida albicans | 99.9% | 30 secs | 0.4 ppm | EN 1650 |
| Bacteriophage | 99.99% | 30 secs | 0.4 ppm | Simulated Use Test |
This Pathogen Summary does not necessarily reflect the claims approved by local, regional, or national regulatory bodies.
This Pathogen Summary does not necessarily reflect the claims approved by local, regional, or national regulatory bodies.
SAO's cleaning efficacy can be supported through adenosine triphosphate (ATP) testing, a widely accepted method for assessing surface hygiene. ATP testing measures organic residue on surfaces and reports results in relative light units (RLU), where lower RLU values indicate cleaner surfaces and higher values indicate greater contamination. The below demonstrates real world ATP results in using SAO.
| Surface | RLU Before SAO | RLU After SAO |
| Lobby Table | 622 | 42 |
| Cafeteria Table | 165 | 2 |
| Elevator Button | 218 | 8 |
| Women's Bathroom | 147 | 0 |
| Door to Plant | 163 | 15 |
| Water Fountain | 58 | 0 |
| Handrail | 122 | 1 |
| Surface | RLU with Chemicals | RLU Before SAO | RLU After SAO |
| Baby Change Table | 345 | 266 | 9 |
| Sanitary Disposal | 6,114 | 28 | 3 |
| Stall Door | 212 | 13 | 2 |
| Surface | RLU Before Chemicals | RLU After Chemicals | RLU Before SAO | RLU After SAO |
| Desk | 107 | 29 | 145 | 8 |
| Pantry | 156 | 143 | 108 | 15 |
| Surface | RLU Before SAO | RLU After SAO |
| Tabletop | 43 | 5 |
| Buffet | 1,195 | 19 |
| Toilet Handle | 144 | 24 |
| Sink Handle | 22 | 4 |
| Elevator Button | 239 | 2 |
Please note, results may vary based on individual client use
Tested, Trusted, and Third-Party Verified
We don’t just claim sustainability—we prove it.
Ecolabels like Green Seal and Nordic Swan maintain some of the strictest standards in the world, requiring rigorous external testing of a product's entire lifecycle, chemical safety, and functional performance. They don't take a manufacturer's word for it; independent auditors verify every detail.
Passing these audits confirms that Stabilized Aqueous Ozone (SAO®) delivers high-performance cleaning without relying on toxic chemicals, synthetic fragrances, or leaving harmful residues behind. Having our technology verified by these leading organizations gives our customers total confidence that they are using a solution that is as safe for people and the planet as it is tough on dirt.
From independent certifications to government approvals, our credentials validate our commitment to delivering safe, effective, and environmentally responsible solutions.
Efficacy, Quality, and Performance
NSF
As certified by the National Sanitation Foundation (NSF), SAO is an approved no-rinse solution for food-contact surfaces, supporting sanitation, and food safety requirements.
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Green Seal
Our Green Seal certifications represent SAO’s third-party verification for efficacy and approval as an industrial cleaning product for commercial and household use.
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Standard
Our technologies are backed by rigorous, third-party scientific testing to validate performance in compliance to AOAC 960.09, ASTM E1153, EN 1276, EN 13697, and EN 14476 standards.
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FDA
The Food and Drug Administration (FDA) authorizes the use of ozone, SAO’s active ingredient, as an antimicrobial agent in the treatment, storage, and processing of food.
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EUO₃TA
As a near-decade long member, our EUO₃TA participation helps validate tersano as an authorized, trusted leader in ozone technology across the entire European market.
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PMRA
The tersano lotus PRO was Canada’s first ozone-generating device to receive approval from the Pest Management Regulatory Agency (PMRA), a division of Health Canada, for safety and efficacy.
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TGA
Since 2024, Australia’s Therapeutic Goods Administration (TGA) has certified tersano’s SAO technology as a recognized disinfectant with validated efficacy.
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WRAS
Water Regulations Approval Scheme (WRAS) certifies that our systems are built to meet the organization's standards for safety, performance, and integration with public water supplies.
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CE
Our technologies are CE certified, representing a public declaration of compliance with essential quality, health, safety, and environmental protection requirements.
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EPA
Our SAO solution aligns with the Environmental Protection Agency's (EPA) ozone regulations.
View StatuteHuman Health and Food Safety
WELL
We comply with WELL standards by enabling healthier indoor environments through chemical-free cleaning that improves air quality and occupant wellbeing.
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OSHA
SAO is Occupational Safety and Health Administration (OSHA) compliant, supporting safer workplace practices by reducing worker exposure to hazardous cleaning chemicals.
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ICC&R
SAO is dermatologically tested and approved for use by individuals with skin sensitivities, reducing irritation risk often associated with traditional cleaning chemicals.
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HACCP
Certified by Hazard Analysis Critical Control Point (HACCP), tersano SAO is approved for use in food primary zones and spill and splash areas to reduce contamination risk.
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USDA Organic
SAO’s active ingredient, ozone, is on the United States Department of Agriculture's (USDA) organic list, supporting safer food-contact cleaning aligned with organic standards.
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CFIA
Our solution meets the Canadian Food Inspection Agency's (CFIA) requirements, supporting compliant use in Canadian regulated food handling and safety environments.
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NSF
Certified by the National Sanitary Foundation (NSF), tersano SAO is an approved no-rinse solution for food-contact surfaces, ensuring safety without chemical residue.
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FDA
Ozone, SAO’s active ingredient, is recognized by the Food and Drug Administration (FDA) as a microbial agent for food applications, supporting safe pathogen control.
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Green Seal
Certified by Green Seal, tersano SAO supports the organization's goals by reducing toxic chemical exposure and promoting healthier human environments.
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EPA
Our SAO solution aligns with the Environmental Protection Agency's (EPA) ozone regulations.
View StatuteEnvironmental Sustainability
LEED
In LEED v5, Stabilized Aqueous Ozone is specifically recognized by the United States Green Building Council as a sustainable cleaning products option supporting LEED accreditation.
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EcoVadis Silver
Our EcoVadis Silver rating reflects strong performance in environmental sustainability and corporate social responsibility across operations and supply chain.
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Green Key
As an official Green Key vendor, tersano supports Green Key standards in the hospitality industry by enabling reduced chemical use and lower environmental impact.
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GECA
Certified by Good Environmental Choice Australia (GECA), tersano supports environmentally preferable products that reduce pollution and promote safer lifecycle impacts.
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Nordic Swan Ecolabel
Available on select formats, Nordic Swan certification validates PROscrub's ability to combine effective performance with responsible environmental practices.
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Green Clean Institute
Certified by Green Cleaning Institute, tersano SAO earned the maximum sustainability assessment points available by supporting sustainable cleaning practices.
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BOMA Best
SAO supports Building Owners and Management Association (BOMA) certification goals by improving building environmental performance through reduced chemical use.
Learn More about BOMA
AASHE
As a business sponsor, tersano supports the Association for the Advancement of Sustainability in Higher Education (AASHE) and the organization's STARS initiative.
Learn More about AASHE
EPA
Our SAO solution aligns with the Environmental Protection Agency's (EPA) ozone regulations.
View Statute
Green Seal
SAO meets Green Seal standards by supporting reduced environmental impact, safer chemical alternatives, and improved lifecycle sustainability in cleaning.
Download CertificateFrom Stanford University to McDonald's, our community of changemakers are sharing their stories on how SAO revolutionized how they clean, work, and operate business.
HSBC
"SAO reaffirms we have used a product that performs better than chemicals in every aspect of the value chain. EFS will deploy tersano SAO for the whole tower."
McDonald's
"We have made the installation and use of tersano SAO mandatory in our new McDonald’s restaurant openings and are very happy to encourage its use."
Stanford University
"It’s almost not even worth calling it green cleaning because it’s so much better. It is just as effective at killing viruses and bacteria as traditional chemical-based cleaners."
AdventHealth
"We simply have fewer chemicals to manage. We even use it on our floors. In the past, we would just be able to clean them. Now we can clean and sanitize them."
Accor Hotels
"The benefits of SAO are many, but if I had to sum it up I would say it’s environmentally-friendly, safer for my staff, and very easy to use."
Toronto Pearson Airport
"We were receiving complaints regularly of strong odors in restrooms. Since our introduction of SAO, complaints about odors at the airport have been extremely reduced.”
F45 Fitness
"SAO is such an easy and affordable way to keep our gym clean. We would never go back to using cleaning chemicals. In fact, whenever I see Lysol wipes, my first response is YUCK!"
Shell Tower
“Using tersano to clean without chemicals is a dream come true. We’re saving over £25,000 a year in reduced chemical usage and labor hours.”
Woodley Acres Pet Resort
"We believe in providing a happy, healthy, and safe place for all our pets. With SAO, we fulfill that mission by removing toxic cleaning chemicals from our facility."
Domino's
"We make hundreds of pizzas a day. Bringing SAO in means we are keeping the cleaning chemicals out: out of our restaurant, out of our ingredients, and out of my staff’s hands."
Looking for more information on our certifications, approvals, lab tests, or case studies? Fill out the contact form below to get in touch with a tersano expert.